• Product NameEGFR (Phospho-Ser1026) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of EGFR only when phosphorylated at serine 1026.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 1026 (P-S-S(p)-P-S) derived from Human EGFR.
• Target NameEGFR
• ModificationPhospho
• Alternative Names    ERBB1; Epidermal growth factor receptor precursor; kinase EGFR;
• Accession No. Swiss-Prot#: P00533;
  NCBI Gene#: 1956;
  NCBI Protein#: NP_005219.2
• UniprotP00533
• Gene ID1956;
• SDS-PAGE MW 160kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Wu SL, et al. (2006)Mol Cell Proteomics 5, 1610-27
Tong J, et al. (2009) Mol Cell Proteomics 8, 2131-44
Daub H, et al. (2008) Mol Cell 31, 438-48

• Western blot analysis of extracts from HeLa cells treated with TSA using Phospho-EGFR (Ser1026) antibody #11902.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting