• Product NamePLCg2(Ab-753) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PLCg2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 751~755 (S-L-Y-D-V) derived from Human PLCg2.
• Target NamePLCg2
• Alternative Names    Phosphoinositide phospholipase C; Phospholipase C-gamma-2;
• Accession No. Swiss-Prot: P16885
  NCBI Protein: NP_002652.2
• UniprotP16885
• Gene ID5336;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 150kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999
Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661
Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029
Rodriguez R, et al. (2003) Biochem J 374 : 269-280

• Western blot analysis of extracts from 293 and 3T3 cells using PLCg2(Ab-753) Antibody #21186.
• Immunofluorescence staining of methanol-fixed Hela cells using PLCg2(Ab-753) Antibody #21186.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting