• Product NameFAK (Phospho-Ser843) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at serine 843.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine843(R-G-S(p)-I-D) derived from Human FAK .
• Target NameFAK
• ModificationPhospho
• Alternative Names    FADK 1; FAK1; Focal adhesion kinase 1; PTK2; pp125FAK
• Accession No. Swiss-Prot#: Q05397;
  NCBI Gene#: 5747;
  NCBI Protein#: NP_001186578.1
• UniprotQ05397
• Gene ID5747;
• SDS-PAGE MW 120kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Jiang X, Sinnett-Smith J, Rozengurt E (2007)Cell Signal 19, 1000-10 . Jacamo R, Jiang X, Lunn JA, Rozengurt E (2007)J Cell Physiol 210, 436-44. Le Boeuf F, Houle F, Sussman M, Huot J (2006)Mol Biol Cell 17, 3508-20 .

• Western blot analysis of extracts from 3T3 cells treated with PMA using Phospho-FAK (Ser843) antibody #11920.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting