• Product Name14-3-3z(Ab-58) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total 14-3-3 z protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.
• Target Name14-3-3z
• Alternative Names    1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1
• Accession No. Swiss-Prot: P63104
  NCBI Protein: NP _001129171.1
• UniprotP63104
• Gene ID7534;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 28kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310
Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387
Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342.

• Western blot analysis of extracts from HepG2 , Hela and 293 cells using 14-3-3z(Ab-58) Antibody #21188.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3z(Ab-58) Antibody #21188(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting