• Product NameATPase (Phospho-Ser16) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of ATPase only when phosphorylated at serine 16.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 16 (A-V-S(p)-E-Q) derived from Human ATPase.
• Target NameATPase
• ModificationPhospho
• Alternative Names    A1A1; AT1A1; ATP1A1; K-ATPase 1; Sodium pump 1
• Accession No. Swiss-Prot#: P05023;
  NCBI Gene#: 476;
  NCBI Protein#: NP_000692.2
• UniprotP05023
• Gene ID476;
• SDS-PAGE MW 112kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Kava L, Rossi NF, Mattingly R, Yingst DR (2012) Am J Hypertens 25, 487-91
Soltoff SP, Asara JM, Hedden L (2010)J Biol Chem 285, 36330-8
Efendiev R, et al. (2006) FEBS Lett 580, 5067-70

• Western blot analysis of extracts from 293 cells treated with PMA using Phospho-ATPase (Ser16) antibody #11938.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting