• Product NameIR (Phospho-Tyr1355) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of IR only when phosphorylated at tyrosine 1355.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1355 (R-S-Y(p)-E-E) derived from Human IR.
• Target NameIR
• ModificationPhospho
• Alternative Names    CD220 antigen; IR; insulin receptor; kinase InsR;
• Accession No. Swiss-Prot#: P06213;
  NCBI Gene#: 3643;
  NCBI Protein#: NP_000199.2
• UniprotP06213
• Gene ID3643;
• SDS-PAGE MW 95kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.

Tennagels N, et al. (2001) Biochem Biophys Res Commun 282, 387-93
Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) FEBS Lett 479, 67-71
Noelle V, Tennagels N, Klein HW (2000) Biochemistry 39, 7170-7

• Western blot analysis of extracts from 293 cells treated with Heatshock using Phospho-IR (Tyr1355) antibody #11939.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting