• Product NameBCL-2 (Phospho-Ser87) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of BCL-2 only when phosphorylated at serine 87.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 87 (A-L-S(p)-P-V) derived from Human BCL-2.
• Target NameBCL-2
• ModificationPhospho
• Alternative Names    Apoptosis regulator Bcl-2; BCL2;
• Accession No. Swiss-Prot#: P10415;
  NCBI Gene#: 596;
  NCBI Protein#: NP_000624.2
• UniprotP10415
• Gene ID596;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Nencioni L, et al. (2009)J Biol Chem 284, 16004-15. Pattingre S, et al. (2009)J Biol Chem 284, 2719-28. Wei Y, et al. (2008)Mol Cell 30, 678-88.

• Western blot analysis of extracts from HeLa cells treated with Nocodazole using Phospho-BCL-2 (Ser87) antibody #11942.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting