• Product NameVE-Cadherin (Phospho-Tyr731) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of VE-Cadherin only when phosphorylated at tyrosine 731.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Tyrosine731 (H-I-Y(p)-G-Y) derived from Human VE-Cadherin.
• Target NameVE-Cadherin
• ModificationPhospho
• Alternative Names    Vascular endothelial cadherin; VE-cadherin; 7B4 antigen; CDH5; CD144 antigen
• Accession No. Swiss-Prot#: P33151;
  NCBI Gene#: 1003;
  NCBI Protein#: NP_001786.2
• UniprotP33151
• Gene ID1003;
• SDS-PAGE MW 130kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen.

Wessel F, et al. (2014) Nat Immunol 15, 223-30
Sawada J, et al. (2012) Cancer Cell 22, 235-49
Turowski P, et al. (2008) J Cell Sci 121, 29-37

• Western blot analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) antibody #11950.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting