• Product NameETK (Phospho-Tyr566) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of ETK only when phosphorylated at tyrosine 566.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Tyrosine566 (D-Q-Y(p)-V-S) derived from Human ETK.
• Target NameETK
• ModificationPhospho
• Alternative Names    BMX; BMX NON-receptor tyrosine kinase; Bone marrow kinase BMX; Epithelial and endothelial tyrosine kinase; NTK38
• Accession No. Swiss-Prot#: P51813;
  NCBI Gene#: 660;
  NCBI Protein#: NP_001712.1
• UniprotP51813
• Gene ID660;
• SDS-PAGE MW 78kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility.

Zhang R, et al. (2003) J Biol Chem 278, 51267-76
Tsai YT, et al. (2000) Mol Cell Biol 20, 2043-54

• Western blot analysis of extracts from HeLa cells treated with Serum using Phospho-ETK (Tyr566) antibody #11953.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting