• Product Name14-3-3 zeta/ delta (Phospho-Thr232) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of 14-3-3 zeta/ delta only when phosphorylated at threonine 232.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine232(S-D-T(p)-Q-G) derived from Human 14-3-3 zeta/ delta .
• Target Name14-3-3 zeta/ delta
• ModificationPhospho
• Alternative Names    1433Z; 143Z; FAS; YWHAZ;
• Accession No. Swiss-Prot#: P63104;
  NCBI Gene#: 7534;
  NCBI Protein#: NP_001129171.1
• UniprotP63104
• Gene ID7534;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Sluchanko NN, et al. (2011) Arch Biochem Biophys 506, 24-34
Sluchanko NN, et al. (2008) Arch Biochem Biophys 477, 305-12
Clokie SJ, et al. (2005) FEBS J 272, 3767-76

• Western blot analysis of extracts from Jurkat cells treated with UV using Phospho-14-3-3 zeta/ delta (Thr232) antibody #11954.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting