• Product NamePKC zeta (Phospho-Thr560) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of PKC zeta only when phosphorylated at threonine 560.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phospho rylation site of threonine560(Q-L-T(p)-P-D) derived from Human PKC zeta .
• Target NamePKC zeta
• ModificationPhospho
• Alternative Names    KPCZ; PKC-zeta; PKC2; PRKCZ; nPKC-zeta
• Accession No. Swiss-Prot#: Q05513;
  NCBI Gene#: 5590;
  NCBI Protein#: NP_001028753.1
• UniprotQ05513
• Gene ID5590;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Nakayama M, et al. (2013)Nat Cell Biol 15, 249-60. Mayanglambam A, Bhavanasi D, Vijayan KV, Kunapuli SP (2011)Biochem Pharmacol 82, 505-13. Dettori R, et al. (2009)J Biol Chem 284, 30318-27.

• Western blot analysis of extracts from COS7 cells treated with PMA using Phospho-PKC zeta (Thr560) antibody #11958.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting