• Product NamePin1 (Phospho-Ser16) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of Pin1 only when phosphorylated at serine 16.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine16(R-M-S(p)-R-S) derived from Human Pin1 .
• Target NamePin1
• ModificationPhospho
• Alternative Names    PPIase Pin1; Rotamase Pin1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; EC 5.2.1.8;
• Accession No. Swiss-Prot#: Q13526;
  NCBI Gene#: 5300;
  NCBI Protein#: NP_006212.1
• UniprotQ13526
• Gene ID5300;
• SDS-PAGE MW 18kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.

Lee YC, et al. (2013)J Cell Sci 126, 4862-72. Ando K, et al. (2013)Neurobiol Aging 34, 757-69. Nechama M, Lin CL, Richter JD (2013)Mol Cell Biol 33, 48-58.

• Western blot analysis of extracts from COS7 cells treated with Insulin using Phospho-Pin1 (Ser16) antibody #11960.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting