• Product NameAMPKα1/AMPKα2 (Ab-183/172) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB,IHC,IF,ELISA
• Species ReactivityHuman,Mouse,Rat,Monkey,Bovine,Fish
• SpecificityThe antibody detects endogenous level of total AMPKa1/AMPKa2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2.
• Target NameAMPKa1/AMPKa2
• Alternative Names    AMPK, AMPKa1
• Accession No. Swiss-Prot: Q13131
  NCBI Protein: NP_006242.5 NP_006243.2
• UniprotQ13131
• Gene ID5562;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 63kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066
Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008
Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641
Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028.

• Western blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-183/172) Antibody #21191.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-183/172) Antibody #21191 (left) or the same antibody preincubated with blocking peptide (right).
• Immunofluorescence staining of methanol-fixed Hela cells using AMPKα1/AMPKα2(Ab-183/172) Antibody #21191.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting