• Product NameAdrenergic Receptor beta2 (Phospho-Ser346) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Adrenergic Receptor beta2 only when phosphorylated at serine 346.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 346 (R-S-S(p)-L-K) derived from Human Adrenergic Receptor beta2.
• Target NameAdrenergic Receptor beta2
• ModificationPhospho
• Alternative Names    ADRB2R; B2AR; adrenergic receptor; beta-2;
• Accession No. Swiss-Prot#: P07550;
  NCBI Gene#: 154;
  NCBI Protein#: NP_000015.1
• UniprotP07550
• Gene ID154;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.

Gao S, Malbon C, Wang HY (2014) J Mol Signal 9, 3
24690384 Curated Info
Vistein R, Puthenveedu MA (2013) Proc Natl Acad Sci U S A 110, 15289-94
Gimenez LE, et al. (2012) J Biol Chem 287, 9028-40

• Western blot analysis of extracts from HepG2 cells treated with Nocodazole using Phospho-Adrenergic Receptor beta2 (Ser346) antibody #11970.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting