• Product Namec-Met (Phospho-Tyr1003) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of c-Met only when phosphorylated at tyrosine 1003.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1003 (V-D-Y(p)-R-A) derived from Human c-Met.
• Target Namec-Met
• ModificationPhospho
• Alternative Names    HGF receptor; HGF-SF receptor; Hepatocyte growth factor receptor precursor; Hepatocyte growth factor receptor precursor; Met proto- oncogene tyros
• Accession No. Swiss-Prot#: P08581;
  NCBI Gene#: 4233;
  NCBI Protein#: NP_000236.2
• UniprotP08581
• Gene ID4233;
• SDS-PAGE MW 155kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Usatyuk PV, et al. (2014)J Biol Chem. Asiedu MK, et al. (2014) Oncogene 33, 1316-24. Dulak AM, et al. (2011) Oncogene 30, 3625-35.

• Western blot analysis of extracts from HepG2 tissue using c-Met (Phospho-Tyr1003) antibody #11971.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting