• Product NameCalmodulin (Phospho-Thr79/Ser81) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Calmodulin only when phosphorylated at threonine 79/serine 81.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine79/serine81(K-D-T(p)-D-S(p)-E-E) derived from Human Calmodulin .
• Target NameCalmodulin
• ModificationPhospho
• Alternative Names    CALM; CALM1; CALM2; CALM3; CAM
• Accession No. Swiss-Prot#: P62158;
  NCBI Gene#: 808;
  NCBI Protein#: NP_005175.2
• UniprotP62158
• Gene ID808;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

Allen D, Fakler B, Maylie J, Adelman JP (2007) J Neurosci 27, 2369-76
Arrigoni G, et al. (2004) Biochemistry 43, 12788-98
Greif DM, Sacks DB, Michel T (2004) Proc Natl Acad Sci U S A 101, 1165-70

• Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using Calmodulin (Phospho-Thr79+Ser81) Antibody. The lane on the right is blocked with the phospho peptide.
• Western Blot analysis of various cells using Phospho-Calmodulin (T80/S82) Polyclonal Antibody
• Immunohistochemistry analysis of paraffin-embedded human brain, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.
• Immunofluorescence analysis of HepG2 cells, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting