• Product NameNMDAR1 (Phospho-Ser890) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB, IH, IF/IC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of NMDAR1 only when phosphorylated at serine 890.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine890(A-S-S(p)-F-K) derived from Human NMDAR1 .
• Target NameNMDAR1
• ModificationPhospho
• Alternative Names    GLURZ1; GRIN1; N-methyl-D-aspartate receptor; NMZ1; NMDZ1
• Accession No. Swiss-Prot#: Q05586;
  NCBI Gene#: 2902;
  NCBI Protein#: NP_000823.4
• UniprotQ05586
• Gene ID2902;
• SDS-PAGE MW 105kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

NMDA receptors are members of the ionotropic class of glutamate receptors, which also includes Kainate and AMPA receptors. NMDA receptors consist of NR1 subunits combined with one or more NR2 (A-D) or NR3 (A-B) subunits. The ligand-gated channel is permeable to cations including Ca2+, and at resting membrane potentials NMDA receptors are inactive due to a voltage-dependent blockade of the channel pore by Mg2+. NMDA receptor activation, which requires binding of glutamate and glycine, leads to an influx of Ca2+ into the postsynaptic region where it activates several signaling cascades, including pathways leading to the induction of long-term potentiation (LTP) and depression (LTD). NMDA receptors have a critical role in excitatory synaptic transmission and plasticity in the CNS. They govern a range of physiological conditions including neurological disorders caused by excitotoxic neuronal injury, psychiatric disorders and neuropathic pain syndromes.

Xu M, Smothers CT, Woodward JJ (2011) Alcohol 45, 373-80. Kong M, Ba M, Song L, Liu Z (2009)Neurochem Res 34, 1513-21 . Jackson MF, et al. (2006)J Neurochem 96, 1509-18.

• Western blot analysis of NMDAR1 (pS890) expression in A549 (A), U87MG (B) whole cell lysates.
• Immunohistochemical analysis of NMDAR1 (pS890) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system.
• Immunofluorescent analysis of NMDAR1 (pS890) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting