• Product NameCaMK4 (Phospho-Thr196/200) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityPhospho-CaMKIV (T200) Polyclonal Antibody detects endogenous levels of CaMKIV protein only when phosphorylated at Thr196/200.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200.
• Target NameCaMK4
• ModificationPhospho
• Alternative Names    CAM kinase-GR; CAMK4; CaMK IV; Calspermin; KCC4
• Accession No. Swiss-Prot#: Q16566;
  NCBI Gene#: 814;
  NCBI Protein#: NP_001735.1
• UniprotQ16566
• Gene ID814;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2).

Oury F, et al. (2010)Genes Dev 24, 2330-42. Dias WB, Cheung WD, Wang Z, Hart GW (2009) J Biol Chem 284, 21327-37. Chow FA, Anderson KA, Noeldner PK, Means AR (2005)J Biol Chem 280, 20530-8.

• Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using CaMK4 (Phospho-Thr196/200) Antibody. The lane on the right is blocked with the phospho peptide.
• Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.
• Immunofluorescence analysis of HeLa cells, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting