• Product NameAurB (Phospho-Thr232) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of AurB only when phosphorylated at threonine 232.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 232 (R-K-T(p)-M-C) derived from Human AurB.
• Target NameAurB
• ModificationPhospho
• Alternative Names    AIK2; AIM-1; ARK2; AURKB; Aurora-B
• Accession No. Swiss-Prot#: Q96GD4;
  NCBI Gene#: 9212;
  NCBI Protein#: NP_001243763.1
• UniprotQ96GD4
• Gene ID9212;
• SDS-PAGE MW 39kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity.

Caldas GV, DeLuca KF, DeLuca JG (2013)J Cell Biol 203, 957-69. Zhang L, et al. (2012)J Biol Chem 287, 34069-77. Ratushny V, et al. (2012)Oncogene 31, 1217-27 .

• Western blot analysis of extracts from COS7 cells treated with Nocodazole using Phospho-AurB (Thr232) antibody #11982.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting