• Product NameGTPase Activating Protein (Phospho-Ser387) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of GTPase Activating Protein only when phosphorylated at serine 387.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 387 (R-I-S(p)-G-C) derived from Human GTPase Activating Protein.
• Target NameGTPase Activating Protein
• ModificationPhospho
• Alternative Names    GTPase activating protein; GTPase activating protein ID-GAP; RAC GTPase- activating protein 1; RACGAP1; RAC GTPase-activating protein
• Accession No. Swiss-Prot#: Q9H0H5;
  NCBI Gene#: 29127;
  NCBI Protein#: NP_001119575.1
• UniprotQ9H0H5
• Gene ID29127;
• SDS-PAGE MW 72kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation.

Doki N, et al. (2009)Cancer Sci 100, 1675-9. Minoshima Y, et al. (2003)Dev Cell 4, 549-60.

• Western blot analysis of extracts from COS7 tissue using GTPase Activating Protein antibody #11983.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemistry analysis of paraffin-embedded human placenta, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.
• Immunofluorescence analysis of HeLa cells, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting