• Product NameHDAC6 (Phospho-Ser22) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of HDAC6 only when phosphorylated at serine 22.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 22 (P-Q-S(p)-P-P) derived from Human HDAC6.
• Target NameHDAC6
• ModificationPhospho
• Alternative Names    HD6; HDA6; Histone deacetylase 6; JM21;
• Accession No. Swiss-Prot#: Q9UBN7;
  NCBI Gene#: 10013;
  NCBI Protein#: NP_006035.2
• UniprotQ9UBN7
• Gene ID10013;
• SDS-PAGE MW 131kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes By similarity. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

Chen S, Owens GC, Makarenkova H, Edelman DB (2010)PLoS One 5, e10848.

• Western blot analysis of extracts from NIH-3T3 tissue using HDAC6 (Phospho-Ser22) antibody #11984.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting