• Product NameVEGFR1 (Phospho-Tyr1213) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of VEGFR1 only when phosphorylated at tyrosine 1213.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Tyrosine 1213(V-R-Y(p)-V-N) derived from Human VEGFR1.
• Target NameVEGFR1
• ModificationPhospho
• Alternative Names    FLT; FLT1; FRT; Flt-1; Fms-like tyrosine kinase 1
• Accession No. Swiss-Prot#: P17948;
  NCBI Gene#: 2321;
  NCBI Protein#: NP_001153392.1
• UniprotP17948
• Gene ID2321;
• SDS-PAGE MW 90kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Raghu H, et al. (2012) Mol Oncol 6, 33-47
Ito N, Huang K, Claesson-Welsh L (2001) Cell Signal 13, 849-54
Ito N, Wernstedt C, Engstr?m U, Claesson-Welsh L (1998) J Biol Chem 273, 23410-8

• Western blot analysis of extracts from HeLa cells treated with UV using Phospho-VEGFR1 (Tyr1213) antibody #11985.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting