• Product NameVitamin D Receptor (Phospho-Ser208) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Vitamin D Receptor only when phosphorylated at serine 208.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 208 (A-L-S(p)-P-V) derived from Human Vitamin D Receptor.
• Target NameVitamin D Receptor
• ModificationPhospho
• Alternative Names    NR1I1; vitamin D receptor; vitamin D3 receptor;
• Accession No. Swiss-Prot#: P11473;
  NCBI Gene#: 7421;
  NCBI Protein#: NP_000367.1
• UniprotP11473
• Gene ID7421;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

Arriagada G, et al. (2007) J Steroid Biochem Mol Biol 103, 425-9. Jurutka PW, et al. (1996)Proc Natl Acad Sci U S A 93, 3519-24. Jurutka PW, et al. (1993)J Biol Chem 268, 6791-9.

• Western blot analysis of extracts from HT29 cells treated with Heatshock using Phospho-Vitamin D Receptor (Ser208) antibody #11994.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting