• Product NameCDK1/CDC2 (Phospho-Thr14) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phospho rylation site of threonine14(E-G-T(p)-Y-G) derived from Human CDK1/CDC2 .
• Target NameCDK1/CDC2
• ModificationPhospho
• Alternative Names    CDC28; CDC2A; CDK1; MPF; kinase Cdc2
• Accession No. Swiss-Prot#: P06493
  NCBI Gene#: 983
  NCBI mRNA#：NM_001170406.1
  NCBI Protein#：NP_001163877.1
• UniprotP06493
• Gene ID983;
• SDS-PAGE MW 34kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.

Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50
Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57

• Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting