• Product NameCCR5 (Phospho-Ser349) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of CCR5 only when phosphorylated at serine 349.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 349 (E-I-S(p)-V-G) derived from Human CCR5.
• Target NameCCR5
• ModificationPhospho
• Alternative Names    C-C CKR-5; CKR5; CHEMR13; CMKBR5; CCR-5
• Accession No. Swiss-Prot#: P51681;
  NCBI Gene#: 1234/727797;
  NCBI Protein#: NP_000570.1.
• UniprotP51681
• Gene ID1234;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.

Martin Oppermann, J. Biol. Chem., Mar 1999; 274: 8875.
Beatrix Pollok-Kopp, J. Biol. Chem., Jan 2003; 278: 2190 - 2198.
Ramtin Rahbar, J. Virol., Jul 2006; 80: 7245 - 7259.
H Olbrich, J. Leukoc. Biol., Mar 1999; 65: 281.

• Western blot analysis of extracts from A549 cells (Lane 2), using CCR5 (Phospho-Ser349) Antibody #11650. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting