• Product NameETS1 (Phospho-Thr38) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of ETS1 only when phosphorylated at threonine 38.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 38(L-L-T(p)-P-S) derived from Human ETS1 .
• Target NameETS1
• ModificationPhospho
• Alternative Names    p54; ETS1; ETS-1; C-ets-1 protein;
• Accession No. Swiss-Prot#: P27577;
  NCBI Gene#: 2113;
  NCBI Protein#: NP_035938.2.
• UniprotP27577
• Gene ID23871;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.

Tsukasa Higuchi, Mol. Cell. Biol., May 2007; 27: 3353 - 3366.
NK Bhat, PNAS, May 1990; 87: 3723.
H Suzuki, PNAS, May 1995; 92: 4442.
V Fafeur, Cell Growth Differ., Jun 1997; 8: 655.

• Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody #11658.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody #11658 (left)or the same antibody preincubated with blocking peptide (right).
• Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody #11658.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting