• Product NameCCDC139 Antibody HRP Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsWB IHC-P IHC-F
• Species ReactivityHu Ms Rt
• Immunogen DescriptionKLH conjugated synthetic peptide derived from human PUS10 CCDC139
• ConjugatesHRP
• Excitation EmissionN A
• Target NameCCDC139
• Alternative Names    CCDC139; Coiled coil domain containing protein 139; DOBI; FLJ32312; MGC126729; Putative tRNA pseudouridine synthase Pus10; tRNA pseudouridine 55 synthase; tRNA uridine isomerase; PUS10_HUMAN.
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200

Pseudouridination is the isomerization of uridine to pseudouridine. It is the most common posttranscriptional nucleotide modification found in RNA. It is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones which facilitate the correct folding and assembly of tRNAs.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting