• Product NameBcr(Ab-177) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total Bcr protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 175~179 (P-F-Y-V-N) derived from Human Bcr.
• Target NameBcr
• Alternative Names    Renal carcinoma antigen NY-REN-26
• Accession No. Swiss-Prot: P11274
  NCBI Protein: NP_004318.3
• UniprotP11274
• Gene ID613;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 130 160 210kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907

• Western blot analysis of extracts from HL60 and K562 cells using Bcr(Ab-177) Antibody #21197.
• Immunofluorescence staining of methanol-fixed Hela cells using Bcr(Ab-177) Antibody #21197.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting