• Product NamePKA α/β CAT (Phospho-Thr197) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 197(T-W-T(p)-L-C) derived from Human PKA α/β CAT.
• Target NamePKA α/β CAT
• ModificationPhospho
• Alternative Names    PRKACA ; PRKACB; PKACA;
• Accession No. Swiss-Prot#: P17612;
  NCBI Gene#: 5566;
  NCBI Protein#: NP_002721.1.
• UniprotP17612
• Gene ID5566;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.
W Li, Learn. Mem., Jan 1996; 2: 320 - 333.
Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.
Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

• Western blot analysis of extracts from mouse brain cells using PKA α/β CAT (Phospho-Thr197) Antibody #11667.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β CAT (Phospho-Thr197) antibody #11667 (left)or the same antibody preincubated with blocking peptide (right).
• Immunofluorescence staining of methanol-fixed A549 cells using PKA α/β CAT (Phospho-Thr197) Antibody #11667.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting