• Product NameACK1 (Phospho-Tyr284) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1.
• Target NameACK1
• ModificationPhospho
• Alternative Names    ACK1; Activated p21cdc42Hs kinase; EC 2.7.10.2; kinase ACK1;
• Accession No. Swiss-Prot#: Q07912;
  NCBI Gene#: 10188;
  NCBI Protein#: NP_005772.3.
• UniprotQ07912
• Gene ID10188;
• SDS-PAGE MW 120kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.

Manser E., Nature 363:364-367(1993).
Ota T., Nat. Genet. 36:40-45(2004).
Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999)

• Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody #11671.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody #11671 (left)or the same antibody preincubated with blocking peptide (right).
• Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody #11671.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting