• Product NameCD32 (Phospho-Tyr292) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 292 (I-T-Y(p)-S-L) derived from Human CD32.
• Target NameCD32
• ModificationPhospho
• Alternative Names    CD32; FCG2; FCG2B,; LY-17; CDW32
• Accession No. Swiss-Prot#: P31994 ;
  NCBI Gene#: 2213;
  NCBI Protein#: NP_003992.3.
• UniprotP31994
• Gene ID2213;
• SDS-PAGE MW 32kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

Stuart S.G., EMBO J. 8:3657-3666(1989).
Brooks D.G., J. Exp. Med. 170:1369-1385(1989).
Engelhardt W., Eur. J. Immunol. 20:1367-1377(1990).

• Western blot analysis of extracts from K562 cells treated with PMA using CD32 (Phospho-Tyr292) Antibody #11672.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting