• Product NamePEA-15 (Phospho-Ser104) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 104(I-P-S(p)-A-K) derived from Human PEA-15.
• Target NamePEA-15
• ModificationPhospho
• Alternative Names    PE15; PEA15; PED;
• Accession No. Swiss-Prot#: Q15121;
  NCBI Gene#: 8682;
  NCBI Protein#: NP_003759.1.
• UniprotQ15121
• Gene ID8682;
• SDS-PAGE MW 19kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

Estelles A., J. Biol. Chem. 271:14800-14806(1996).
Condorelli G., EMBO J. 17:3858-3866(1998).
Wolford J.K., Gene 241:143-148(2000).

• Western blot analysis of extracts from COS cells treated with TNF using PEA-15 (Phospho-Ser104) Antibody #11676.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody #11676 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting