• Product NameRas-GRF1 (Phospho-Ser916) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1.
• Target NameRas-GRF1
• ModificationPhospho
• Alternative Names    CDC25; GNRP; GRF1; RASGRF1;
• Accession No. Swiss-Prot#: Q13972;
  NCBI Gene#: 5923;
  NCBI Protein#: NP_002882.3.
• UniprotQ13972
• Gene ID5923;
• SDS-PAGE MW 145kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.
Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.
Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.
Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

• Immunohistochemical analysis of paraffin-embedded Human kidney cancer using Ras-GRF1 (Phospho-Ser916) antibody #11678.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting