• Product NameB-RAF (Phospho-Thr599) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 599 (L-A-T(p)-V-K) derived from Human B-RAF.
• Target NameB-RAF
• ModificationPhospho
• Alternative Names    B-RAF; RAFB1; RMIL; BRAF1; c-RMIL
• Accession No. Swiss-Prot#: P15056;
  NCBI Gene#: 673;
  NCBI Protein#: NP_004324.2.
• UniprotP15056
• Gene ID673;
• SDS-PAGE MW 84kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• WB dilution: 1:500-1:1000
  Immunohistochemistry: 1:50~1:100
  IF dilution: 1:100-1:200

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418.
Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949.
R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953.
Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using B-Raf (Phospho-Thr598) antibody #11682 (left)or the same antibody preincubated with blocking peptide (right).
• Western blot analysis of extracts from mouse brain/rat lung, using Phospho-B-RAF (Thr599) Antibody. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide)
• Immunofluorescence staining of methanol-fixed HeLa cells using B-RAF (Phospho-Thr599) Antibody #11682.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting