• Product NameBAD (Phospho-Ser91/128) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of BAD only when phosphorylated at serine 91/ serine 128.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 128 (E-L-S(p)-P-F) derived from Mouse BAD.
• Target NameBAD
• ModificationPhospho
• Alternative Names    BAD; BBC6; BCL2L8; Bcl- XL/Bcl-2 associated death promoter;
• Accession No. Swiss-Prot#: Q92934;
  NCBI Gene#: 572;
  NCBI Protein#: NP_004313.1.
• UniprotQ92934
• Gene ID572;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Wang H.-G., Cell 87:629-638(1996).
Ottilie S., J. Biol. Chem. 272:30866-30872(1997).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody #11685.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody #11685 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting