• Product NamePaxillin(Ab-31) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Paxillin protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin.
• Target NamePaxillin
• Alternative Names    PAXI; PXN;
• Accession No. Swiss-Prot: P49023
  NCBI Protein: NP_001074324.1
• UniprotP49023
• Gene ID5829;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 68kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426
Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939
Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128
Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626
Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454

• Western blot analysis of extracts from 293 cells using Paxillin(Ab-31) Antibody #21199.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin(Ab-31) Antibody #21199(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting