• Product NameHistamine H1 Receptor (Phospho-Ser398) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of Histamine H1 Receptor only when phosphorylated at serine 398.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 398(S-H-S(p)-R-Q) derived from Human Histamine H1 Receptor .
• Target NameHistamine H1 Receptor
• ModificationPhospho
• Alternative Names    H1-R; HRH1; histamine H1 receptor;
• Accession No. Swiss-Prot#: P35367;
  NCBI Gene#: 3269;
  NCBI Protein#: NP_000852.1.
• UniprotP35367
• Gene ID3269;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunofluorescence: 1:100~1:200

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.

de Backer M.D., Biochem. Biophys. Res. Commun. 197:1601-1608(1993).
Fukui K., Biochem. Biophys. Res. Commun. 201:894-901(1994).
Sjoeblom T., Science 314:268-274(2006)

• Immunofluorescence staining of methanol-fixed HuvEc cells using Histamine H1 Receptor (Phospho-Ser398) Antibody #11695.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting