• Product NameITGB4 (Phospho-Tyr1510) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHuman,Rat,Mouse
• SpecificityThe antibody detects endogenous levels of ITGB4 only when phosphorylated at tyrosine 1510.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine1510(R-D-Y(p)-S-T) derived from Human ITGB4 .
• Target NameITGB4
• ModificationPhospho
• Alternative Names    GP150; ITB4; CD104 antigen;
• Accession No. Swiss-Prot#: P16144;
  NCBI Gene#: 3691;
  NCBI Protein#: NP_000204.3.
• UniprotP16144
• Gene ID3691;
• SDS-PAGE MW 202kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.

Suzuki S., EMBO J. 9:757-763(1990).
Hogervorst F., EMBO J. 9:765-770(1990).
Tamura R.N., J. Cell Biol. 111:1593-1604(1990).

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ITGB4 (Phospho-Tyr1510) antibody #11698 (left)or the same antibody preincubated with blocking peptide (right).
• Western blot analysis of extracts from HepG2 cells treated with Na2VO3 using ITGB4 (Phospho-Tyr1510) Antibody #11698.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting