• Product NamePAK1 (Phospho-Ser199) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of PAK1/2 only when phosphorylated at serine 199.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 199(T-K-S(p)-V-I) derived from Human PAK1.
• Target NamePAK1
• ModificationPhospho
• Alternative Names    ADRB2; PAK 1; P65-PAK; P68-PAK;
• Accession No. Swiss-Prot#: Q13153;
  NCBI Gene#: 5058;
  NCBI Protein#: NP_002567.3.
• UniprotQ13153
• Gene ID5058;
• SDS-PAGE MW 61kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Brown J.L., Curr. Biol. 6:598-605(1996).
Sells M.A., Curr. Biol. 7:202-210(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody #11699.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody #11699 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting