• Product NameTGF β Receptor II (Phospho-Ser225/250) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of TGF β Receptor II only when phosphorylated at serine 225/250.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 225/250(D-R-S(p)-D-I) derived from Human TGF β Receptor II.
• Target NameTGF β Receptor II
• ModificationPhospho
• Alternative Names    TGF-betaR2; TGFR2; kinase TGF-beta-R2;
• Accession No. Swiss-Prot#: P37173;
  NCBI Gene#: 7048;
  NCBI Protein#: NP_003233.4.
• UniprotP37173
• Gene ID7048;
• SDS-PAGE MW 64kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis.

RC Tripathi, Invest. Ophthalmol. Vis. Sci., Jan 1993; 34: 260.
N Chegini, Endocrinology, Jul 1994; 135: 439.
GW Mulheron, Endocrinology, Dec 1992; 131: 2609

• Immunohistochemical analysis of paraffin-embedded human brain tissue using TGF β Receptor II (Phospho-Ser225/250) antibody #11700 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting