• Product NameIGF2R (Phospho-Ser2409) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of IGF2R only when phosphorylated at serine 2409.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 2409(Q-D-S(p)-E-D) derived from Human IGF2R.
• Target NameIGF2R
• ModificationPhospho
• Alternative Names    CI-MPR; CI-MPR; MPR300; MPRI;
• Accession No. Swiss-Prot#: P11717;
  NCBI Gene#: 3482;
  NCBI Protein#: NP_000867.2.
• UniprotP11717
• Gene ID3482;
• SDS-PAGE MW 300kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.

Morgan D.O., Nature 329:301-307(1987).
Oshima A., J. Biol. Chem. 263:2553-2562(1988).
Killian J.K., Mamm. Genome 10:74-77(1999)

• Western blot analysis of extracts from COS-7 cells treated with UV using IGF2R (Phospho-Ser2409) Antibody #11708.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human brain tissue using IGF2R (Phospho-Ser2409) antibody #11708 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting