• Product Namec-Jun (Phospho-Ser63) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe Antibody detects endogenous levels of c-Jun only when phosphorylated at Serine 63.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 63(L-T-S(p)-P-D) derived from Human c-Jun.
• Target Namec-Jun
• ModificationPhospho
• Alternative Names    AH119; c-Jun; p39; Jun A; AP1
• Accession No. Swiss-Prot#: P05412;
  NCBI Gene#: 3725;
  NCBI Protein#: NP_002219.1.
• UniprotP05412
• Gene ID3725;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.
Sevilla A, et al. (2004). Oncogene.23(55): 8950-8958.
Li L, et al. (2004). J Biol Chem.279(6): 4058-4065.
Hurd C, et al. (2002). Oncogene.21(14): 2154-2160.
 

• Western blot analysis of extracts from HeLa cells using c-Jun (Phospho-Ser63) Antibody #11710.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using c-Jun (Phospho-Ser63) antibody #11710 (left)or the same antibody preincubated with blocking peptide (right).
• Western blotting analysis using c-Jun (Phospho-Ser63) Antibody #11710.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting