• Product NameeNOS (Phospho-Thr495) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 495 (K-K-T(p)-F-K derived from Human eNOS .
• Target NameeNOS
• ModificationPhospho
• Alternative Names    cNOS; EC-NOS; ECNOS; NOS; NOS3
• Accession No. Swiss-Prot#: P29474;
  NCBI Gene#: 4846;
  NCBI Protein#: NP_000594.2.
• UniprotP29474
• Gene ID4846;
• SDS-PAGE MW 140kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Janssens S.P., J. Biol. Chem. 267:14519-14522(1992).
Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
Marsden P.A., FEBS Lett. 307:287-293(1992)

• Western blot analysis of extracts from Jurkat cells and HepG2 cells using eNOS (Phospho-Thr495) Antibody #11711.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting