• Product NameIGF1R (Phospho-Tyr1346) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of IGF1R only when phosphorylated at tyrosine 1346.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1346(Q-P-Y(p)-A-H) derived from Human IGF1R .
• Target NameIGF1R
• ModificationPhospho
• Alternative Names    CD221; kinase IGF1R; IGF1R;
• Accession No. Swiss-Prot#: P08069;
  NCBI Gene#: 3480;
  NCBI Protein#: NP_000866.1.
• UniprotP08069
• Gene ID3480;
• SDS-PAGE MW 154kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

Ullrich A., EMBO J. 5:2503-2512(1986).
Abbot A.M., J. Biol. Chem. 267:10759-10763(1992).
The MGC Project Team;Genome Res. 14:2121-2127(2004).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using IGF1R (Phospho-Tyr1346) antibody #11716 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting