• Product Namep53 (Phospho-Thr387) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of p53 only when phosphorylated at threonine 387.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 387(F-K-T(p)-E-G) derived from Human p53.
• Target Namep53
• ModificationPhospho
• Alternative Names    TP53; tumor protein p53; Tumor suppressor p53;
• Accession No. Swiss-Prot#: P04637;
  NCBI Gene#: 7157;
  NCBI Protein#: NP_000537.3.
• UniprotP04637
• Gene ID7157;
• SDS-PAGE MW 43kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Zakut-Houri R., EMBO J. 4:1251-1255(1985).
Lamb P., Mol. Cell. Biol. 6:1379-1385(1986).
Harlow E., Mol. Cell. Biol. 5:1601-1610(1985).

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Thr387) antibody #11718 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting