• Product NameRetinoblastoma (Phospho-Ser608) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 608.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 608(Y-L-S(p)-P-V) derived from Human Retinoblastoma.
• Target NameRetinoblastoma
• ModificationPhospho
• Alternative Names    P105-RB; PP105; PP110; RB1;
• Accession No. Swiss-Prot#: P06400;
  NCBI Gene#: 5925;
  NCBI Protein#: NP_000312.2.
• UniprotP06400
• Gene ID5925;
• SDS-PAGE MW 110kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  IF dilution: 1:100-1:200

Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.

Lee W.-H., Nature 329:642-645(1987).
Lee W.-H., Science 235:1394-1399(1987).
Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987).

• Western blot analysis of extracts from MCF7 cells, using Phospho-Rb (Ser608) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.
• Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Retinoblastoma (Phospho-Ser608) antibody #11720.
• Immunofluorescence staining of methanol-fixed Hela cells using Retinoblastoma (Phospho-Ser608) Antibody #11720.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting