• Product NameSTMN1 (Phospho-Ser63) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of STMN1 only when phosphorylated at serine 63.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 63(R-K-S(p)-H-E) derived from Human STMN1.
• Target NameSTMN1
• ModificationPhospho
• Alternative Names    LAG; LAP18; pp19; PR22; STN1
• Accession No. Swiss-Prot#: P16949;
  NCBI Gene#: 3925;
  NCBI Protein#: NP_005554.1.
• UniprotP16949
• Gene ID3925;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Alternatively spliced transcript variants encoding the same protein have been identified.

Zhu X.-X., J. Biol. Chem. 264:14556-14560(1989).
Maucuer A., FEBS Lett. 264:275-278(1990).
Melhem R.F., J. Biol. Chem. 266:17747-17753(1991).

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STMN1 (Phospho-Ser63) antibody #11722 (left)or the same antibody preincubated with blocking peptide (right).
• Western blot analysis of extracts from COS cells treated with nocodazole using STMN1 (Phospho-Ser63) Antibody #.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting