• Product NameABL1 (Phospho-Thr735) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of ABL1 only when phosphorylated at threonine 735.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine735 (S-V-T(p)-L-P) derived from Human ABL1.
• Target NameABL1
• ModificationPhospho
• Alternative Names    Abelson murine leukemia viral oncogene 1; ABL1; c-ABL; JTK7; p150
• Accession No. Swiss-Prot#: P00519;
  NCBI Gene#: 25;
  NCBI Protein#: NP_005148.2.
• UniprotP00519
• Gene ID25;
• SDS-PAGE MW 135kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

Fainstein E., Oncogene 4:1477-1481(1989).
Shtivelman E., Cell 47:277-284(1986).
Chissoe S.L., Genomics 27:67-82(1995).

• Western blot analysis of extracts from COS cells treated with EGF using ABL1 (Phospho-Thr735) Antibody #11725.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting