• Product NameALK (Phospho-Tyr1096) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1096.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1096 (P-N-Y(p)-C-F) derived from Human ALK.
• Target NameALK
• ModificationPhospho
• Alternative Names    ALK tyrosine kinase receptor; anaplastic lymphoma kinase; anaplastic lymphoma kinase (Ki-1); CD246; EC 2.7.10.1; kinase ALK
• Accession No. Swiss-Prot#: Q9UM73;
  NCBI Gene#: 238;
  NCBI Protein#: NP_004295.2
• UniprotQ9UM73
• Gene ID238;
• SDS-PAGE MW 176kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain.

Morris S.W., Oncogene 14:2175-2188(1997).
Morris S.W., Oncogene 15:2883-2883(1997).
Iwahara T., Oncogene 14:439-449(1997).

• Immunohistochemical analysis of paraffin-embedded human brain tissue, using ALK (Phospho-Tyr1096) antibody #11726 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting