• Product NameBLK (Phospho-Tyr501) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of BLK only when phosphorylated at tyrosine 501.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 501 (R-Q-Y(p)-E-L) derived from Human BLK.
• Target NameBLK
• ModificationPhospho
• Alternative Names    p55-BLK; EC 2.7.10.2; kinase Blk; B lymphocyte kinase; Tyrosine-protein kinase BLK
• Accession No. Swiss-Prot#: P51451;
  NCBI Gene#: 640;
  NCBI Protein#: NP_001706.2.
• UniprotP51451
• Gene ID640;
• SDS-PAGE MW 57kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor.

Islam K.B., J. Immunol. 154:1265-1272(1995).
Drebin J.A.,Oncogene 10:477-486(1995).
Greenman C.,Nature 446:153-158(2007).

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody #11727 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting